Journal: PLoS ONE
Article Title: Kidins220/ARMS Is a Novel Modulator of Short-Term Synaptic Plasticity in Hippocampal GABAergic Neurons
doi: 10.1371/journal.pone.0035785
Figure Lengend Snippet: A ) Immunofluorescence images of a glutamatergic (vGlut-positive) and a GABAergic (vGAT-positive) autaptic neuron stained with anti-Kidins220 (red), anti-vGlut1 (green) and anti-vGAT (blue) antibodies. Merged images are shown on the right. Scale bars, 10 µm. B ) Higher magnification of excitatory and inhibitory neuronal processes stained with anti-Kidins220 (red) and anti-vGlut or anti-vGAT (green) antibodies. Merged images are shown on the right. Kidins220 shows a good co-localization with both pre-synaptic markers (arrowheads), indicating pre-synaptic localization of the protein in both excitatory and inhibitory terminals. Scale bars, 1 µm.
Article Snippet: The following primary antibodies were used: monoclonal anti-Kidins220 , polyclonal anti-vesicular GABA transporter (vGAT, AB5062P, Millipore), polyclonal anti-vesicular Glutamate transporter 1 (vGlut1, AB5905 Millipore).
Techniques: Immunofluorescence, Staining